Dolichyl-phosphate-mannose--protein mannosyltransferase 5 (PMT5), partial, Saccharomyces cerevisiae,

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Source: E.coli. Expression Region: 286-583aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SVHIKTLNVN GISSSFFPAE FRKTLKYNNV IKETVAEVAV GSAVSLNHVG TAGGYLHSHL HNYPAGSMQQ QVTLYPHIDQ NNKWIIELAE HPNENVTSFQ NLTDGTIIKL RQLKNGCRLH SHDHKPPVSQ NADWQKEVSC YGYEGFEGDI NDDWIIEIDK KRSEPGPAQE HIRAIETKFR LKHYLTGCYL FSHPEKLPEW GFGQQEVTCA YFAREDLTSW YIEENENEIS LPNPEKVSYK KMSFWQKFVA IHKFMFYLNN YMDTSHAYSS EPKTWPLMLR GIDFWNENGR EVYFLGNA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Protein O-mannosyltransferase involved in O-glycosylation which is essential for cell wall rigidity. Forms a heterodimeric complex with PMT3 and more rarely with PMT2 to transfer mannose from Dol-P-mannose to Ser or Thr residues on proteins. Reference: "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames." Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A. Yeast 12:1377-1384(1996). Function: nan