DNase I, RNase-free, GMP-Grade DMF #038032

Product Description DNase I is an endonuclease that digests single- and double-stranded DNA to oligonucleotide containing a 5' phosphate and 3'-hydroxylated ends. It is an essential enzyme for the efficient digestion of DNA during RNA purification and is suitable for molecular diagnostics and in vitro mRNA synthesis applications. This product has been submitted to the FDA Drug Master Files and is assigned DMF #038032. Concentration: 4U/µL, Unit Definition: One unit corresponds to the amount of enzyme required to produce a change in absorbance at 260 nm of 1.0 in 10 minutes. Source: Expressed in an E.coli strain with DNase I gene isolated from bovine pancreas. Quality Standards: Activity (Degradation plasmid method): > 4 kU/mL, Purity (SEC-HPLC): > 95%, Residual RNase: Negative, Residual Protease: Negative, Endotoxin: <= 1.2 EU/mL, Residual Host Cell DNA: <= 100 pg/mg, Residual Host protein: <= 20 ng/mg, Residual Heavy Metal: <= 10 ppm, Residual Nickel Salt: <= 10 ppm, Bioburden: <= 1 CFU/10 mL.