DNA polymerase II large subunit (polC), partial, Cenarchaeum symbiosum, recombinant

Organism: Cenarchaeum symbiosum (strain A). Source: E.coli. Expression Region: 287-832aa. Protein Length: Partial. Tag Info: Tag-Free. Target Protein Sequence: LAELKGAVQT GENKEDAAAK RMREVITGRS VLSMPNRLGG FRLRYGRACN TGYTSVGFHP AVAEILDHTI AVGTQVKIDI PGKGATVAFV DTIEAPTVRL AGGDVVKIRD VAHGIELKGS IERILHLGDM LISFGDFLEN NAQLVPSGYV EEIWKMDMEA AGAAQGSPSS ADEAVRISRE LGVPLHPRYL YYWDQISHEE LAMLLSPLDK GDAISYPAAC KPVLEKLGVP HKAGPEGPVL EGDEARIFRE LILDNPPGPD ASAPVPELIS RSSGITIRDK FSTSIGVRIG RPEKAAPRQM RPPTHCLFPV GGTGGPTNNL LKSAARPGFS ADILSRRCPG CGEPSISIRC WACGERTAVE RTCMQCGTDV DGEECERCGR PGLAHSRVEF PLKKMLVSAQ EKTGVRAHDP LKGVKELAHQ DRIAEPLEKG LIRQSRSLTV FKDGTVRFDA TNSPMTHFKP SWIGTSAEKL RELGYETDVD GKKLEGPDQL VELRMQDIVI PLEGAKYLVS ACGYIDAELD KLYGAPPFYK VPDLGGLIGH LVVGLA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase. Reference: "Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum." Hallam S.J., Konstantinidis K.T., Putnam N., Schleper C., Watanabe Y., Sugahara J., Preston C., de la Torre J., Richardson P.M., DeLong E.F. Proc. Natl. Acad. Sci. U.S.A. 103:18296-18301(2006). Function: Possesses two activities