DNA polymerase I, thermostable (polA), partial, recombinant Thermus aquaticus

Organism: Thermus aquaticus. Source: E.coli. Expression Region: 310-832aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: VLSRKEPMWA DLLALAAARG GRVHRAPEPY KALRDLKEAR GLLAKDLSVL ALREGLGLPP GDDPMLLAYL LDPSNTTPEG VARRYGGEWT EEAGERAALS ERLFANLWGR LEGEERLLWL YREVERPLSA VLAHMEATGV RLDVAYLRAL SLEVAEEIAR LEAEVFRLAG HPFNLNSRDQ LERVLFDELG LPAIGKTEKT GKRSTSAAVL EALREAHPIV EKILQYRELT KLKSTYIDPL PDLIHPRTGR LHTRFNQTAT ATGRLSSSDP NLQNIPVRTP LGQRIRRAFI AEEGWLLVAL DYSQIELRVL AHLSGDENLI RVFQEGRDIH TETASWMFGV PREAVDPLMR RAAKTINFGV LYGMSAHRLS QELAIPYEEA QAFIERYFQS FPKVRAWIEK TLEEGRRRGY VETLFGRRRY VPDLEARVKS VREAAERMAF NMPVQGTAAD LMKLAMVKLF PRLEEMGARM LLQVHDELVL EAPKERAEAV ARLAKEVMEG VYPLAVPLEV EVGIGEDWLS AKE. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: n/a. Reference: Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus.Lawyer F.C., Stoffel S., Saiki R.K., Myambo K., Drummond R., Gelfand D.H.J. Biol. Chem. 264:6427-6437(1989). Function: nan