Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochond

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 86-642aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SLPPHQKVPL PSLSPTMQAG TIARWEKKEG EKISEGDLIA EVETDKATVG FESLEECYMA KILVPEGTRD VPVGSIICIT VEKPQDIEAF KNYTLDLAAA AAPQAAPAAA PAPAAAPAAP SASAPGSSYP THMQIVLPAL SPTMTMGTVQ RWEKKVGEKL SEGDLLAEIE TDKATIGFEV QEEGYLAKIL VPEGTRDVPL GAPLCIIVEK QEDIAAFADY RPTEVTSLKP QAAPPAPPPV AAVPPTPQPV APTPSAAPAG PKGRVFVSPL AKKLAAEKGI DLTQVKGTGP EGRIIKKDID SFVPSKAAPA AAAAMAPPGP RVAPAPAGVF TDIPISNIRR VIAQRLMQSK QTIPHYYLSV DVNMGEVLLV RKELNKMLEG KGKISVNDFI IKASALACLK VPEANSSWMD TVIRQNHVVD VSVAVSTPAG LITPIVFNAH IKGLETIASD VVSLASKARE GKLQPHEFQG GTFTISNLGM FGIKNFSAII NPPQACILAI GASEDKLIPA DNEKGFDVAS VMSVTLSCDH RVVDGAVGAQ WLAEFKKYLE KPITMLL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle. Reference: "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways." Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y. Mol. Cell 50:919-930(2013). Function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.