Diacetylchitobiose deacetylase 14A, Bacillus cereus

Diacetylchitobiose deacetylase 14A (BcChe14A), assigned the E.C. number 3.5.1.-, is a derivative of Bacillus cereus. It is an enzyme that exhibits deacetylase activity with the N-acetyl moiety of the N-acetylglucosamine and the diacetylchitobiose and triacetylchitotriose. The recombinant BcChe14A, purified from Escherichia coli, is a single-domain Carbohydrate Esterase family 14 (CE14) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Carbohydrate Esterase. Activity: Diacetylchitobiose deacetylases. Family: CE14. Ec Number: 3.5.1.-. Organism: Bacillus cereus. Architecture: CE14. Optimal pH: 8. Optimal Temperature: 37°C. Substrates: diacetylchitobiose (GlcNAc2), N-acetylglucosamine (GlcNAc), triacetylchitobiose (GlcNAc3)