Dha hydrolase 143A & beta-L-Arabinofuranosidase 142A, Bacteroides thetaiotaomicron

Dha hydrolase 143A & beta-L-Arabinofuranosidase 142A (BtDha143A-Hyp142A), assigned the E.C. number 3.2.1.- and 3.2.1.185, is a derivative of Bacteroides thetaiotaomicron. It is a bifunctional 2-keto-3-deoxy-D-lyxo-heptulosaric acid (Dha) hydrolase and beta-L-arabinofuranosidase. The recombinant BtDha143A-Hyp142A, purified from Escherichia coli, is a modular Glycoside Hydrolase family 143 and 142 (GH143 and GH142) enzyme, with a C-terminal family 143 Glycoside Hydrolase (GH143) (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Other Activities & Arabinofuranosidases. Family: GH142, GH143. Ec Number: 3.2.1.- and 3.2.1.185. Organism: Bacteroides thetaiotaomicron. Architecture: GH143-GH142. Optimal pH: 7. Optimal Temperature: 37°C. Substrates: rhamnogalacturonan II