Desmoglein-3 (Dsg3), partial (Active), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 50-617aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: EWVKFAKPCR EREDNSRRNP IAKITSDFQK NQKITYRISG VGIDQPPFGI FVVDPNNGDI NITAIVDREE TPSFLITCRA LNALGQDVER PLILTVKILD VNDNPPIFSQ TIFKGEIEEN SASNSLVMIL NATDADEPNH MNSKIAFKIV SQEPAGMSMF LISRNTGEVR TLTSSLDREQ ISSYHLVVSG ADNDGTGLST QCECSIKIKD VNDNFPVLRE SQYSARIEEN TLNAELLRFQ VTDWDEEYTD NWLAVYFFTS GNEGNWFEIE TDPRTNEGIL KVVKALDYEQ VQSMQFSIAV RNKAEFHQSV ISQYRVQSTP VTIQVIDVRE GISFRPPSKT FTVQRGVSTN KLVGYILGTY QATDEDTGKA ASSVRYVLGR NDGGLLVIDS KTAQIKFVKN IDRDSTFIVN KTISAEVLAI DENTGKTSTG TIYVEVPSFN ENCPSVVLEK KDICTSSPSV TLSVRTLDRG KYTGPYTVSL EEQPLKLPVM WTITTLNATS ALLQAQQQVS PGVYNVPVIV KDNQDGLCDT PESLTLTVCQ CDDRSMCRAP IPSREPNTYG ESSWRLGP. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Dsg3 at 5 µg/mL can bind Anti-DSG3 recombinant antibody (CSB-RA007205A0HU), the EC50 is 2.624-3.182 µg/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Reference: n/a. Function: nan