Desmoglein-1 (DSG1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 50-548aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: EWIKFAAACR EGEDNSKRNP IAKIHSDCAA NQQVTYRISG VGIDQPPYGI FVINQKTGEI NITSIVDREV TPFFIIYCRA LNSMGQDLER PLELRVRVLD INDNPPVFSM ATFAGQIEEN SNANTLVMIL NATDADEPNN LNSKIAFKII RQEPSDSPMF IINRNTGEIR TMNNFLDREQ YGQYALAVRG SDRDGGADGM SAECECNIKI LDVNDNIPYM EQSSYTIEIQ ENTLNSNLLE IRVIDLDEEF SANWMAVIFF ISGNEGNWFE IEMNERTNVG ILKVVKPLDY EAMQSLQLSI GVRNKAEFHH SIMSQYKLKA SAISVTVLNV IEGPVFRPGS KTYVVTGNMG SNDKVGDFVA TDLDTGRPST TVRYVMGNNP ADLLAVDSRT GKLTLKNKVT KEQYNMLGGK YQGTILSIDD NLQRTCTGTI NINIQSFGND DRTNTEPNTK ITTNTGRQES TSSTNYDTST TSTDSSQVYS SEPGNGAKDL LSDNVHFGP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Reference: "Desmosomal glycoprotein DGI, a component of intercellular desmosome junctions, is related to the cadherin family of cell adhesion molecules."Wheeler G.N., Parker A.E., Thomas C.L., Ataliotis P., Poynter D., Arnemann J., Rutman A.J., Pidsley S.C., Watt F.M., Rees D.A., Buxton R.S., Magee A.I.Proc. Natl. Acad. Sci. U.S.A. 88:4796-4800(1991). Function: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.