Deoxyribonuclease I (DNase I), 2u/ul, Solution (EC 3.1.21.1, 5'-Oligonucleotidohydrolase)

Deoxyribonuclease I (DNase I), 2u/ul, Solution (EC 3.1.21.1, 5'-Oligonucleotidohydrolase)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
D3200-02.100 100 U - -

3 - 19 Werktage*

374,00 €
D3200-02.500 500 U - -

3 - 19 Werktage*

548,00 €
 
Endonuclease which digests dsDNA by hydrolysis of deoxyribonucleotide linkages, producing... mehr
Produktinformationen "Deoxyribonuclease I (DNase I), 2u/ul, Solution (EC 3.1.21.1, 5'-Oligonucleotidohydrolase)"
Endonuclease which digests dsDNA by hydrolysis of deoxyribonucleotide linkages, producing 3'-hydroxyl oligonucleotides. Applications: Nick Translation, DNase I DNA Footprinting, Cloning of Random DNA Fragments, Activity: ~2000u/ml, Unit Definition: 1 unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 5.0. Assay Method: Based on the Kunitz method where DNase will depolymerize DNA, 1 Kunitz units digests 1ug of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Ca+2 and 1mM Mg+2, pH 7.8 in a 50ul reaction. Correlation of digestion units with Kunitz is different for other DNA and buffer systems, Quality Control: SDS-PAGE, RNase: None Detected, Protease: None Detected, Storage and Stability: May be stored at 4°C for short-term only. Protect from moisture. For long-term storage, store at -20°C. Aliquots with 50% glycerol are stable for 6 months at -20°C. , , Note: Pancreatic DNase is highly susceptible to denaturation. Mix by gentle inversion.
Hersteller: United States Biological
Hersteller-Nr: D3200-02

Eigenschaften

Konjugat: No
Format: Molecular Biology

Datenbank Information

CAS : 9003-98-9| Passende Produkte

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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