Dedicator of cytokinesis protein 8 (Dock8), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1633-2067aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: KSYQASPDLR LTWLQNMAEK HTKKKCFTEA AMCLVHAAAL VAEYLSMLED HSYLPVGSVS FQNISSNVLE ESAVSDDTLS PDEDGVCSGR YFTESGLVGL LEQAAELFST GGLYETVNEV YKLVIPILEA HRDFRKLTST HDKLQKAFDN IINKDHKRMF GTYFRVGFYG SRFGDLDEQE FVYKEPAITK LPEISHRLEG FYGQCFGAEF VEVIKDSTPV DKTKLDPNKA YIQITFVEPY FDEYEMKDRV TYFEKNFNLR RFMYTTPFTL EGRPRGELHE QHRRNTVLTT MHAFPYIKTR IRVSQKEEFV LTPIEVAIED MKKKTLQLAV ATHQEPPDAK MLQMVLQGSV GATVNQGPLE VAQVFLAEIP ADPKLYRHHN KLRLCFKEFI MRCGEAVEKN RRLITAEQRE YQQELKKNYN KLRDSLRPMI ERKIP. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Guanine nucleotide exchange factor which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP. During immune responses, required for interstitial dendritic cell migration by locally activating CDC42 at the leading edge membrane of DC. Required for CD4+ T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane. Is involved in NK cell cytotoxicity controlling polarization of microtubule-organizing center, and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing. Reference: "Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs." Okazaki Y., Furuno M., Kasukawa T., Adachi J., Bono H., Kondo S., Nikaido I., Osato N., Saito R., Suzuki H., Yamanaka I., Kiyosawa H., Yagi K., Tomaru Y., Hasegawa Y., Nogami A., Schonbach C., Gojobori T. Hayashizaki Y. Nature 420:563-573(2002). Function: Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP