D-glucuronyl C5-epimerase (GLCE), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 29-617aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: NKCSSDKAIQ FPRRSSSGFR VDGFEKRAAA SESNNYMNHV AKQQSEEAFP QEQQKAPPVV GGFNSNVGSK VLGLKYEEID CLINDEHTIK GRREGNEVFL PFTWVEKYFD VYGKVVQYDG YDRFEFSHSY SKVYAQRAPY HPDGVFMSFE GYNVEVRDRV KCISGVEGVP LSTQWGPQGY FYPIQIAQYG LSHYSKNLTE KPPHIEVYET AEDRDKNKPN DWTVPKGCFM ANVADKSRFT NVKQFIAPET SEGVSLQLGN TKDFIISFDL KFLTNGSVSV VLETTEKNQL FTIHYVSNAQ LIAFKERDIY YGIGPRTSWS TVTRDLVTDL RKGVGLSNTK AVKPTKIMPK KVVRLIAKGK GFLDNITIST TAHMAAFFAA SDWLVRNQDE KGGWPIMVTR KLGEGFKSLE PGWYSAMAQG QAISTLVRAY LLTKDHIFLN SALRATAPYK FLSEQHGVKA VFMNKHDWYE EYPTTPSSFV LNGFMYSLIG LYDLKETAGE KLGKEARSLY ERGMESLKAM LPLYDTGSGT IYDLRHFMLG IAPNLARWDY HTTHINQLQL LSTIDESPVF KEFVKRWKSY LKGSRAKHN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins. Reference: Uncovering a biphasic catalytic mode of C5-epimerase in heparan sulfate biosynthesis.Sheng J., Xu Y., Dulaney S.B., Huang X., Liu J.J. Biol. Chem. 287:20996-21002(2012). Function: Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins.