Cytotoxic T-lymphocyte associated protein 4 (CTLA4), partial (Active), guinea pig, recombinant

Organism: Cavia porcellus (Guinea pig). Source: Yeast. Expression Region: 37-161aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: AMHVAQPAVV LASSRGVASF ECEYASSHNA NEVRVTVLQQ VASRTTEICA ATYTVERELA FPEDSACAGT SSGTRVNLTI QGLRAADTGL YICKVELMYP PPYFVGTGNG TQIYVIDPEP CPDSD. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/µg as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse B7-1-Fc at 5 µg/ml can bind Cavia porcellus CTLA-4-His, the ED50 of Recombinant Cavia porcellus CTLA-4-His is 4.29 ng/ml. Form: Liquid. Buffer: 0.2 µm filtered 1xPBS, pH 7.4. Reconstitution: n/a. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cytotoxic Tlymphocyte 4(CTLA-4,CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily.CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4. Reference: n/a. Function: nan