Cytoskeleton-associated protein 4 (Ckap4), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 109-575aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: VLEEVQQVRR GHQDFSRQRD ELGQGLQGVE QKVQSLQATF GTFESLLRNS QHKQDLTEKA VKEGESELNR ISEVLQKLQN EILKDLSDGI HVVKDARERD FTSLENTVEE RLTELTKSIN DNIAIFTDVQ KRSQKEINEV KMKVASLEES KGDRSQDVKT LKDAVKEVQA SMMSRERDIE ALKSSLQTME SDVYTEVREL VSLKQEQQAF KQAADSERLA LQALTEKLLR SEESSSRLPE DIRRLEEELQ QLKVGAHGSE EGAVFKDSKA LEELQRQIEG LGARLQYVED GVYSMQVASA RHTESLESLL SKSQEYEQRL AMLQEHVGNL GSSSDLASTV RSLGETQLAL SSDLKELKQS LGELPGTVES LQEQVLSLLS QDQAQAEGLP PQDFLDRLSS LDNLKSSVSQ VESDLKMLRT AVDSLVAYSV KIETNENNLE SAKGLLDDLR NDLDRLFLKV EKIHEKI. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: High-affinity epithelial cell surface receptor for APF. Mediates the anchoring of the endoplasmic reticulum to microtubules. Reference: "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways." Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y. Mol. Cell 50:919-930(2013). Function: High-affinity epithelial cell surface receptor for APF.