Cysteine protease-like VirA (virA), Shigella flexneri, recombinant

Organism: Shigella flexneri. Source: E.coli. Expression Region: 1-400aa. Protein Length: Full Length. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: MQTSNITNHE RNDSSWMSTV KSTTEVSWNK LSFCDILLKI ITFGIYSPHE TLAEKHSEKK LMDSFSPSLS QDKMDGEFAH ANIDGISIRL CLNKGICSVF YLDGDKIQST QLSSKEYNNL LSSLPPKQFN LGKVHTITAP VSGNFKTHKP APEVIETAIN CCTSIIPNDD YFHVKDTDFN SVWHDIYRDI RASDSNSTKI YFNNIEIPLK LIADLINELG INEFIDSKKE LQMLSYNQVN KIINSNFPQQ DLCFQTEKLL FTSLFQDPAF ISALTSAFWQ SLHITSSSVE HIYAQIMSEN IENRLNFMPE QRVINNCGHI IKINAVVPKN DTAISASGGR AYEVSSSILP SHITCNGVGI NKIETSYLVH AGTLPSSEGL RNAIPPESRQ VSFAIISPDV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks. Creates a tunnel inside the host cell cytoplasm by breaking down the microtubule infrastructure. This facilitates the bacterium's movement through the cytoplasm and also helps other bacteria move faster during the invasion of the eukaryotic cell. Is absolutely required for virulence. Reference: n/a. Function: nan