Collagen alpha-3 (VI) chain (COL6A3), partial, human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 2853-3176aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: HKQVNVPNNV TSSPTSNPVT TTKPVTTTKP VTTTTKPVTT TTKPVTIINQ PSVKPAAAKP APAKPVAAKP VATKMATVRP PVAVKPATAA KPVAAKPAAV RPPAAAAAKP VATKPEVPRP QAAKPAATKP ATTKPMVKMS REVQVFEITE NSAKLHWERA EPPGPYFYDL TVTSAHDQSL VLKQNLTVTD RVIGGLLAGQ TYHVAVVCYL RSQVRATYHG SFSTKKSQPP PPQPARSASS STINLMVSTE PLALTETDIC KLPKDEGTCR DFILKWYYDP NTKSCARFWY GGCGGNENKF GSQKECEKVC APVLAKPGVI SVMG. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Collagen VI acts as a cell-binding protein. Reference: "Mosaic structure of globular domains in the human type VI collagen alpha 3 chain: similarity to von Willebrand factor, fibronectin, actin, salivary proteins and aprotinin type protease inhibitors." Chu M.-L., Zhang R.-Z., Pan T.-C., Stokes D., Conway D., Kuo H.-J., Glanville R., Mayer U., Mann K., Deutzmann R., Timpl R. EMBO J. 9:385-393(1990). Function: nan