Cobalamin import ATP-binding protein BtuD (btuD), Halobacterium salinarum, recombinant

Organism: Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1). Source: E.coli. Expression Region: 1-398aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MTLDVTGLDV ELAGTRILDD VHASIRDGHL VGVVGPNGAG KSTLLRAMNG LITPTAGTVL VAGDDVHALS SAAASRRIAT VPQDASVSFE FTVRQVVEMG RHPHTTRFGT DTDTAVVDRA MARTGVAQFA ARDVTSLSGG ERQRVLLARA LAQAAPVLLL DEPTASLDVN HQIRTLEVVR DLADSEDRAV VAAIHDLDLA ARYCDELVVV ADGRVHDAGA PRSVLTPDTI RAAFDARVAV GTDPATGAVT VTPLPDRTSA AADTSVHVVG GGDSATPVVR RLVSAGASVS VGPVVEGDTD HETARRVGCP CTSVAPFTRL EDTTAASATR ADIAAADVIA VPVAAAARPG VRGLLTGAVP TLAVGDAAGA PEWADRLVAC DAVVSAVGAL ADTPSDGV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin import. Probably responsible for energy coupling to the transport system. Reference: "Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008). Function: Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably responsible for energy coupling to the transport system (By similarity).