Coagulation factor XII (F12), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 20-372aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: IPPWEAPKEH KYKAEEHTVV LTVTGEPCHF PFQYHRQLYH KCTHKGRPGP QPWCATTPNF DQDQRWGYCL EPKKVKDHCS KHSPCQKGGT CVNMPSGPHC LCPQHLTGNH CQKEKCFEPQ LLRFFHKNEI WYRTEQAAVA RCQCKGPDAH CQRLASQACR TNPCLHGGRC LEVEGHRLCH CPVGYTGAFC DVDTKASCYD GRGLSYRGLA RTTLSGAPCQ PWASEATYRN VTAEQARNWG LGGHAFCRNP DNDIRPWCFV LNRDRLSWEY CDLAQCQTPT QAAPPTPVSP RLHVPLMPAQ PAPPKPQPTT RTPPQSQTPG ALPAKREQPP SLTRNGPLSC GQRLRKSLSS MTR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. Reference: "Histidine-rich glycoprotein binds factor XIIa with high affinity and inhibits contact-initiated coagulation." Macquarrie J.L., Stafford A.R., Yau J.W., Leslie B.A., Vu T.T., Fredenburgh J.C., Weitz J.I. Blood 117:4134-4141(2011). Function: nan