Coagulation factor VIII (F8), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-216aa. Protein Length: Full Length of Isoform 2. Tag Info: N-terminal 6xHis-GST-tagged. Target Protein Sequence: MRIQDPGKVF FGNVDSSGIK HNIFNPPIIA RYIRLHPTHY SIRSTLRMEL MGCDLNSCSM PLGMESKAIS DAQITASSYF TNMFATWSPS KARLHLQGRS NAWRPQVNNP KEWLQVDFQK TMKVTGVTTQ GVKSLLTSMY VKEFLISSSQ DGHQWTLFFQ NGKVKVFQGN QDSFTPVVNS LDPPLLTRYL RIHPQSWVHQ IALRMEVLGC EAQDLY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa. Reference: "The acidic region of the factor VIII light chain and the C2 domain together form the high affinity binding site for von Willebrand factor." Saenko E.L., Scandella D. J. Biol. Chem. 272:18007-18014(1997). Function: nan