Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1), Biotinylated, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 40-521aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. Target Protein Sequence: ISTRSPRPFN EIPSPGDNGW LNLYHFWRET GTHKVHLHHV QNFQKYGPIY REKLGNVESV YVIDPEDVAL LFKSEGPNPE RFLIPPWVAY HQYYQRPIGV LLKKSAAWKK DRVALNQEVM APEATKNFLP LLDAVSRDFV SVLHRRIKKA GSGNYSGDIS DDLFRFAFES ITNVIFGERQ GMLEEVVNPE AQRFIDAIYQ MFHTSVPMLN LPPDLFRLFR TKTWKDHVAA WDVIFSKADI YTQNFYWELR QKGSVHHDYR GILYRLLGDS KMSFEDIKAN VTEMLAGGVD TTSMTLQWHL YEMARNLKVQ DMLRAEVLAA RHQAQGDMAT MLQLVPLLKA SIKETLRLHP ISVTLQRYLV NDLVLRDYMI PAKTLVQVAI YALGREPTFF FDPENFDPTR WLSKDKNITY FRNLGFGWGV RQCLGRRIAE LEMTIFLINM LENFRVEIQH LSDVGTTFNL ILMPEKPISF TFWPFNQEAT QQ. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Reference: Analysis of the DNA sequence and duplication history of human chromosome 15.Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.Nature 440:671-675(2006). Function: nan