CES2 (human, recombinant)

Carboxylesterase 2 (CES2) is a serine hydrolase with a major role in endo- and xenobiotic metabolism. It exists as a monomer and is composed of an alpha/beta-hydrolase fold, a regulatory domain, a catalytic domain, and an HTEL endoplasmic reticulum (ER) localization sequence at the C-terminus. CES2 is mainly expressed in liver, kidney, and small intestine, but is also found in cardiovascular and reproductive tissues. It is involved in the metabolism of several xenobiotics, including anticancer prodrugs and cocaine, and also has triacylglycerol and diacylglycerol hydrolase activity. Generally, CES2 prefers to hydrolyze esters with a large alcohol moiety and a small acyl group. Knockdown of CES2 decreases fatty acid oxidation, glucose uptake, and glycogen synthesis and increases the expression of genes involved in gluconeogenesis and ER stress in primary human hepatocytes. Hepatic overexpression of Ces2 decreases hepatic triglyceride and cholesterol levels in db/db mice and in a mouse model of obesity induced by a high-fat diet. Liver levels of CES2 are decreased in patients with obesity or non-alcoholic steatohepatitis (NASH) and tumoral levels of CES2 decrease with increasing tumor grade in patients with colorectal cancer. Cayman's CES2 (human, recombinant) protein can be used for enzyme activity assays. This protein consists of 544 amino acids, has a calculated molecular weight of 60.4 kDa, and a predicted N-terminus of Gln27 after signal peptide cleavage.Synonyms: Carboxylesterase 2, CE-2, Cocaine Esterase, Methylumbelliferyl-acetate Deacetylase 2. Purity: >95% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg determined by the LAL endotoxin assay. Source: Recombinant human C-terminal His-tagged CES2 expressed in HEK293 cells. Amino Acids: 27-559. MW: 60.4 kDa. Formulation: (Request formulation change), Lyophilized from sterile 50 mM sodium acetate, with 150 mM sodium chloride, and 10% glycerol, pH 5.5. Host: HEK293 cells. UniProt Accession #: O00748-1.