Ceroid-lipofuscinosis neuronal protein 5 (CLN5), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 47-358aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: IPSRRHWPVP YKRFDFRPKP DPYCQAKYTF CPTGSPIPVM EGDDDIEVFR LQAPVWEFKY GDLLGHLKIM HDAIGFRSTL TGKNYTMEWY ELFQLGNCTF PHLRPEMDAP FWCNQGAACF FEGIDDVHWK ENGTLVQVAT ISGNMFNQMA KWVKQDNETG IYYETWNVKA SPEKGAETWF DSYDCSKFVL RTFNKLAEFG AEFKNIETNY TRIFLYSGEP TYLGNETSVF GPTGNKTLGL AIKRFYYPFK PHLPTKEFLL SLLQIFDAVI VHKQFYLFYN FEYWFLPMKF PFIKITYEEI PLPIRNKTLS GL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Plays a role in influencing the retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from the endosomes to the trans-Golgi network by controlling the recruitment of retromer complex to the endosomal membrane. Regulates the localization and activation of RAB7A which is required to recruit the retromer complex to the endosomal membrane. Reference: "The role of ceroid lipofuscinosis neuronal protein 5 (CLN5) in endosomal sorting." Mamo A., Jules F., Dumaresq-Doiron K., Costantino S., Lefrancois S. Mol. Cell. Biol. 32:1855-1866(2012). Function: nan