Cellulase 5B, Bacillus halodurans

Cellulase 5B (BhCel5B), assigned the E.C. number 3.2.1.4, is a derivative of Bacillus halodurans. It is an endo-1,4-beta-glucanase. The recombinant BhCel5B, purified from Escherichia coli, is a modular Glycoside Hydrolase family 5 (GH5) enzyme, with a C-terminal family 46 Carbohydrate Binding Module (CBM46) (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Cellulases. Family: GH5. Ec Number: 3.2.1.4. Organism: Bacillus halodurans. Architecture: GH5-CBM46. Optimal pH: 7.5. Optimal Temperature: 60°C. Substrates: 1,3-1,4-beta-glucans, 1,4-beta-glucans, xyloglucan