CEACAM8/CD66b (human, recombinant)

CD66b, also known as carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), is a member of the carcinoembryonic antigen (CEA) family encoded by CEACAM8 in humans. It is a single-chain glycoprotein, comprised of N-terminal immunoglobulin (Ig) variable-like (IgV) and Ig constant-like (IgC) domains, that is anchored to the cell membrane by a glycosylphosphatidylinositol (GPI) linker. CD66b is expressed exclusively in granulocytes, with neutrophil and eosinophil expression increasing under inflammatory conditions to mediate intercellular adhesion to endothelial cells, signal transduction, and cell activation. The percentage of colonic CD66b+ neutrophils is increased and positively correlates with endoscopic disease severity in patients with Crohn's disease. The percentage of CD66b+ neutrophils is also increased in gastric cancer tissues and is associated with poor prognosis. Cayman's CEACAM8/CD66b (human, recombinant) protein can be used for binding assays. The protein consists of 296 amino acids, has a calculated molecular weight of 32.8 kDa, and a predicted N-terminus of Gln35 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 55 to 60 kDa due to glycosylation.Synonyms: Carcinoembryonic Antigen CGM6, Carcinoembryonic Antigen-related Cell Adhesion Molecule 8, CGM6, NCA-95, Non-specific Cross-reacting Antigen NCA-95. Purity: >96% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg, determined by the LAL endotoxin assay. Source: Active recombinant human C-terminal His-tagged CD66b expressed in HEK293 cells. Amino Acids: 35-319. MW: 32.8 kDa. Formulation: (Request formulation change), Lyophilized from sterile PBS, pH 7.4. UniProt Accession #: P31997.