CCR4-NOT transcription complex subunit 1 (CNOT1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 604-867aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: KIREHGEPFI QACMTFLKRR CPSILGGLAP EKDQPKSAQL PPETLATMLA CLQACAGSVS QELSETILTM VANCSNVMNK ARQPPPGVMP KGRPPSASSL DAISPVQIDP LAGMTSLSIG GSAAPHTQSM QGFPPNLGSA FSTPQSPAKA FPPLSTPNQT TAFSGIGGLS SQLPVGGLGT GSLTGIGTGA LGLPAVNNDP FVQRKLGTSG LNQPTFQQSK MKPSDLSQVW PEANQHFSKE IDDEANSYFQ RIYNHPPHPT MSVD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity. Reference: n/a. Function: nan