CCAAT/enhancer-binding protein delta (CEBPD), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-269aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SAALFSLDGP ARGAPWPAEP APFYEPGRAG KPGRGAEPGA LGEPGAAAPA MYDDESAIDF SAYIDSMAAV PTLELCHDEL FADLFNSNHK AGGAGPLELL PGGPARPLGP GPAAPRLLKR EPDWGDGDAP GSLLPAQVAA CAQTVVSLAA AGQPTPPTSP EPPRSSPRQT PAPGPAREKS AGKRGPDRGS PEYRQRRERN NIAVRKSRDK AKRRNQEMQQ KLVELSAENE KLHQRVEQLT RDLAGLRQFF KQLPSPPFLP AAGTADCR. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Transcription activator that recognizes two different DNA motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Transcriptional activator that enhances IL6 transcription alone and as heterodimer with CEBPB. Reference: "Signal transducer and activator of transcription 3 activates CCAAT enhancer-binding protein delta gene transcription in G0 growth-arrested mouse mammary epithelial cells and in involuting mouse mammary gland." Hutt J.A., O'Rourke J.P., DeWille J. J. Biol. Chem. 275:29123-29131(2000). Function: nan