Biosynthetic arginine decarboxylase (speA), partial, Escherichia coli, recombinant

Organism: Escherichia coli (strain K12). Source: E.coli. Expression Region: 3-656aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: DDMSMGLPSS AGEHGVLRSM QEVAMSSQEA SKMLRTYNIA WWGNNYYDVN ELGHISVCPD PDVPEARVDL AQLVKTREAQ GQRLPALFCF PQILQHRLRS INAAFKRARE SYGYNGDYFL VYPIKVNQHR RVIESLIHSG EPLGLEAGSK AELMAVLAHA GMTRSVIVCN GYKDREYIRL ALIGEKMGHK VYLVIEKMSE IAIVLDEAER LNVVPRLGVR ARLASQGSGK WQSSGGEKSK FGLAATQVLQ LVETLREAGR LDSLQLLHFH LGSQMANIRD IATGVRESAR FYVELHKLGV NIQCFDVGGG LGVDYEGTRS QSDCSVNYGL NEYANNIIWA IGDACEENGL PHPTVITESG RAVTAHHTVL VSNIIGVERN EYTVPTAPAE DAPRALQSMW ETWQEMHEPG TRRSLREWLH DSQMDLHDIH IGYSSGIFSL QERAWAEQLY LSMCHEVQKQ LDPQNRAHRP IIDELQERMA DKMYVNFSLF QSMPDAWGID QLFPVLPLEG LDQVPERRAV LLDITCDSDG AIDHYIDGDG IATTMPMPEY DPENPPMLGF FMVGAYQEIL GNMHNLFGDT EAVDVFVFPD GSVEVELSDE GDTVADMLQY VQLDPKTLLT QFRDQVKKTD LDAELQQQFL EEFEAGLYGY TYLE. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the biosynthesis of agmatine from arginine. Reference: Nucleotide sequence and analysis of the speA gene encoding biosynthetic arginine decarboxylase in Escherichia coli.Moore R.C., Boyle S.M.J. Bacteriol. 172:4631-4640(1990). Function: Catalyzes the biosynthesis of agmatine from arginine.