Astacin-like metalloendopeptidase (ASTL), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 24-431aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: APLASSCAGA CGTSFPDGLT PEGTQASGDK DIPAINQGLI LEETPESSFL IEGDIIRPSP FRLLSATSNK WPMGGSGVVE VPFLLSSKYD EPSRQVILEA LAEFERSTCI RFVTYQDQRD FISIIPMYGC FSSVGRSGGM QVVSLAPTCL QKGRGIVLHE LMHVLGFWHE HTRADRDRYI RVNWNEILPG FEINFIKSQS SNMLTPYDYS SVMHYGRLAF SRRGLPTITP LWAPSVHIGQ RWNLSASDIT RVLKLYGCSP SGPRPRGRGS HAHSTGRSPA PASLSLQRLL EALSAESRSP DPSGSSAGGQ PVPAGPGESP HGWESPALKK LSAEASARQP QTLASSPRSR PGAGAPGVAQ EQSWLAGVST KPTVPSSEAG IQPVPVQGSP ALPGGCVPRN HFKGMSED. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding. Reference: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." Bailey S.D., Xie C., Do R., Montpetit A., Diaz R., Mohan V., Keavney B., Yusuf S., Gerstein H.C., Engert J.C., Anand S. Diabetes Care 33:2250-2253(2010). Function: Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding (By similarity).