Aspartic proteinase nepenthesin-2 (nep2), recombinant Nepenthes gracilis

Organism: Nepenthes gracilis (Slender pitcher plant). Source: E.coli. Expression Region: 80-438aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-KSI-tagged. Target Protein Sequence: QSSSGIETPV YAGDGEYLMN VAIGTPDSSF SAIMDTGSDL IWTQCEPCTQ CFSQPTPIFN PQDSSSFSTL PCESQYCQDL PSETCNNNEC QYTYGYGDGS TTQGYMATET FTFETSSVPN IAFGCGEDNQ GFGQGNGAGL IGMGWGPLSL PSQLGVGQFS YCMTSYGSSS PSTLALGSAA SGVPEGSPST TLIHSSLNPT YYYITLQGIT VGGDNLGIPS STFQLQDDGT GGMIIDSGTT LTYLPQDAYN AVAQAFTDQI NLPTVDESSS GLSTCFQQPS DGSTVQVPEI SMQFDGGVLN LGEQNILISP AEGVICLAMG SSSQLGISIF GNIQQQETQV LYDLQNLAVS FVPTQCGAS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Extracellular proteinase found in the pitcher fluid of carnivorous plants. Digest prey for nitrogen uptake. Reference: n/a. Function: nan