Asialoglycoprotein receptor 2 (Asgr2), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: in vitro E.coli expression system. Expression Region: 1-301aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MEKDFQDIQQ LDSEENDHQL IGDEEQGSHV QNLRTENPRW GGQPPSRPFP QRLCSKFRLS LLALAFNILL LVVICVVSSQ SMQLQKEFWT LKETLSNFST TTLMEFKALD SHGGSRNDNL TSWETILEKK QKDIKADHST LLFHLKHFPL DLRTLTCQLA FFLSNGTECC PVNWVEFGGS CYWFSRDGLT WAEADQYCQM ENAHLLVINS REEQEFVVKH RGAFHIWIGL TDKDGSWKWV DGTEYRSNFK NWAFTQPDNW QGHEEGGSED CAEILSDGLW NDNFCQQVNR WACERKRDIT Y. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface. Reference: "Fatty acylation of the rat and human asialoglycoprotein receptors. A conserved cytoplasmic cysteine residue is acylated in all receptor subunits." Zeng F.Y., Weigel P.H. J. Biol. Chem. 271:32454-32460(1996). Function: nan