Asialoglycoprotein receptor 1 (Asgr1), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Mammalian cell. Expression Region: 61-284aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: QNSQLREDLR VLRQNFSNFT VSTEDQVKAL TTQGERVGRK MKLVESQLEK HQEDLREDHS RLLLHVKQLV SDVRSLSCQM AALRGNGSER ICCPINWVEY EGSCYWFSSS VKPWTEADKY CQLENAHLVV VTSWEEQRFV QQHMGPLNTW IGLTDQNGPW KWVDGTDYET GFKNWRPGQP DDWYGHGLGG GEDCAHFTTD GHWNDDVCRR PYRWVCETEL GKAN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface. Reference: "Rat liver asialoglycoprotein receptor lacks a cleavable NH2-terminal signal sequence." Holland E.C., Leung J.O., Drickamer K. Proc. Natl. Acad. Sci. U.S.A. 81:7338-7342(1984). Function: nan