Apoptin (VP3), Chicken anemia virus, recombinant

Organism: Chicken anemia virus (isolate Germany Cuxhaven-1) (CAV). Source: E.coli. Expression Region: 1-121aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MNALQEDTPP GPSTVFRPPT SSRPLETPHC REIRIGIAGI TITLSLCGCA NARAPTLRSA TADNSESTGF KNVPDLRTDQ PKPPSKKRSC DPSEYRVSEL KESLITTTPS RPRTAKRRIR L. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May act as transcriptional regulator. Induces apoptosis in infected cells. Element of infectious replication cycle. Reference: "Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle."Noteborn M.H.M., de Boer G.F., van Roozelaar D.J., Karreman C., Kranenburg O., Vos J.G., Jeurissen S.H.M., Hoeben R.C., Zantema A., Koch G., van Ormondt H., van der Eb A.J.J. Virol. 65:3131-3139(1991). Function: May act as transcriptional regulator. Induces apoptosis in infected cells. Element of infectious replication cycle.