Annexin A4 (ANXA4), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-319aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. Target Protein Sequence: ATKGGTVKAA SGFNAMEDAQ TLRKAMKGLG TDEDAIISVL AYRNTAQRQE IRTAYKSTIG RDLIDDLKSE LSGNFEQVIV GMMTPTVLYD VQELRRAMKG AGTDEGCLIE ILASRTPEEI RRISQTYQQQ YGRSLEDDIR SDTSFMFQRV LVSLSAGGRD EGNYLDDALV RQDAQDLYEA GEKKWGTDEV KFLTVLCSRN RNHLLHVFDE YKRISQKDIE QSIKSETSGS FEDALLAIVK CMRNKSAYFA EKLYKSMKGL GTDDNTLIRV MVSRAEIDML DIRAHFKRLY GKSLYSFIKG DTSGDYRKVL LVLCGGDD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Reference: "Placental anticoagulant proteins: isolation and comparative characterization four members of the lipocortin family." Tait J.F., Sakata M., McMullen B.A., Miao C.H., Funakoshi T., Hendrickson L.E., Fujikawa K. Biochemistry 27:6268-6276(1988). Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.