Annexin A2 (Anxa2), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 2-339aa. Protein Length: Full Length. Tag Info: C-terminal hFc-tagged. Target Protein Sequence: STVHEILCKL SLEGDHSTPP SAYGSVKPYT NFDAERDALN IETAVKTKGV DEVTIVNILT NRSNVQRQDI AFAYQRRTKK ELPSALKSAL SGHLETVILG LLKTPAQYDA SELKASMKGL GTDEDSLIEI ICSRTNQELQ EINRVYKEMY KTDLEKDIIS DTSGDFRKLM VALAKGRRAE DGSVIDYELI DQDARELYDA GVKRKGTDVP KWISIMTERS VCHLQKVFER YKSYSPYDML ESIKKEVKGD LENAFLNLVQ CIQNKPLYFA DRLYDSMKGK GTRDKVLIRI MVSRSEVDML KIRSEFKRKY GKSLYYYIQQ DTKGDYQKAL LYLCGGDD. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response . Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 . Reference: "Annexin A2 is a natural extrahepatic inhibitor of the PCSK9-induced LDL receptor degradation." Seidah N.G., Poirier S., Denis M., Parker R., Miao B., Mapelli C., Prat A., Wassef H., Davignon J., Hajjar K.A., Mayer G. PLoS ONE 7:E41865-E41865(2012). Function: nan