Allene oxide synthase, chloroplastic (CYP74A), Arabidopsis thaliana, recombinant

Organism: Arabidopsis thaliana (Mouse-ear cress). Source: E.coli. Expression Region: 34-518aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SGSETPDLTV ATRTGSKDLP IRNIPGNYGL PIVGPIKDRW DYFYDQGAEE FFKSRIRKYN STVYRVNMPP GAFIAENPQV VALLDGKSFP VLFDVDKVEK KDLFTGTYMP STELTGGYRI LSYLDPSEPK HEKLKNLLFF LLKSSRNRIF PEFQATYSEL FDSLEKELSL KGKADFGGSS DGTAFNFLAR AFYGTNPADT KLKADAPGLI TKWVLFNLHP LLSIGLPRVI EEPLIHTFSL PPALVKSDYQ RLYEFFLESA GEILVEADKL GISREEATHN LLFATCFNTW GGMKILFPNM VKRIGRAGHQ VHNRLAEEIR SVIKSNGGEL TMGAIEKMEL TKSVVYECLR FEPPVTAQYG RAKKDLVIES HDAAFKVKAG EMLYGYQPLA TRDPKIFDRA DEFVPERFVG EEGEKLLRHV LWSNGPETET PTVGNKQCAG KDFVVLVARL FVIEIFRRYD SFDIEVGTSP LGSSVNFSSL RKASF. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalytic activity (13S)-hydroperoxy-(9Z,11E,15Z)-octadecatrienoate = (9Z,13S,15Z)-12,13-epoxyoctadeca-9,11,15-trienoate + H2O Pathway: oxylipin biosynthesis This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism. Reference: "Design and synthesis of novel imidazole derivatives as potent inhibitors of allene oxide synthase(CYP74)." Oh K., Murofushi N. Bioorg. Med. Chem. 10:3707-3711(2002). Function: nan