Aldehyde dehydrogenase 1A1 (Aldh1a1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 2-501aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SSPAQPAVPA PLADLKIQHT KIFINNEWHN SVSGKKFPVL NPATEEVICH VEEGDKADVD KAVKAARQAF QIGSPWRTMD ASERGRLLNK LADLMERDRL LLATMEALNG GKVFANAYLS DLGGCIKALK YCAGWADKIH GQTIPSDGDI FTYTRREPIG VCGQIIPWNF PMLMFIWKIG PALSCGNTVV VKPAEQTPLT ALHLASLIKE AGFPPGVVNI VPGYGPTAGA AISSHMDVDK VAFTGSTQVG KLIKEAAGKS NLKRVTLELG GKSPCIVFAD ADLDIAVEFA HHGVFYHQGQ CCVAASRIFV EESVYDEFVK RSVERAKKYV LGNPLTPGIN QGPQIDKEQH DKILDLIESG KKEGAKLECG GGRWGNKGFF VQPTVFSNVT DEMRIAKEEI FGPVQQIMKF KSVDDVIKRA NNTTYGLAAG LFTKDLDKAI TVSSALQAGV VWVNCYMMLS AQCPFGGFKM SGNGRELGEH GLYEYTELKT VAMKISQKNS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: In addition to the activity on acetaldehyde and related substrates, is also involved in the oxidation of aldehydes derived from biogenic amines such as epinephrine and norepinephrine, as well as the aldehydes generated via lipid peroxidation. Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Reference: "Isolation and characterization of a cytosolic aldehyde dehydrogenase-encoding cDNA from mouse liver."Rongnoparut P., Weaver S.Gene 101:261-265(1991). Function: Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (By similarity).