Adseverin (SCIN), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-715aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MARELYHEEF ARAGKQAGLQ VWRIEKLELV PVPQSAHGDF YVGDAYLVLH TAKTSRGFTY HLHFWLGKEC SQDESTAAAI FTVQMDDYLG GKPVQNRELQ GYESNDFVSY FKGGLKYKAG GVASGLNHVL TNDLTAKRLL HVKGRRVVRA TEVPLSWDSF NKGDCFIIDL GTEIYQWCGS SCNKYERLKA NQVATGIRYN ERKGRSELIV VEEGSEPSEL IKVLGEKPEL PDGGDDDDII ADISNRKMAK LYMVSDASGS MRVTVVAEEN PFSMAMLLSE ECFILDHGAA KQIFVWKGKD ANPQERKAAM KTAEEFLQQM NYSKNTQIQV LPEGGETPIF KQFFKDWRDK DQSDGFGKVY VTEKVAQIKQ IPFDASKLHS SPQMAAQHNM VDDGSGKVEI WRVENNGRIQ VDQNSYGEFY GGDCYIILYT YPRGQIIYTW QGANATRDEL TTSAFLTVQL DRSLGGQAVQ IRVSQGKEPV HLLSLFKDKP LIIYKNGTSK KGGQAPAPPT RLFQVRRNLA SITRIVEVDV DANSLNSNDV FVLKLPQNSG YIWVGKGASQ EEEKGAEYVA SVLKCKTLRI QEGEEPEEFW NSLGGKKDYQ TSPLLETQAE DHPPRLYGCS NKTGRFVIEE IPGEFTQDDL AEDDVMLLDA WEQIFIWIGK DANEVEKKES LKSAKMYLET DPSGRDKRTP IVIIKQGHEP PTFTGWFLGW DSSKW. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: Not Test. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Ca(2+)-dependent actin filament-severing protein that has a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. Severing activity is inhibited by phosphatidylinositol 4,5-bis-phosphate (PIP2). In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Required for megakaryocyte differentiation, maturation, polyploidization and apoptosis with the release of platelet-like particles. Plays a role in osteoclastogenesis (OCG) and actin cytoskeletal organization in osteoclasts. Regulates chondrocyte proliferation and differentiation. Inhibits cell proliferation and tumorigenesis. Signaling is mediated by MAPK, p38 and JNK pathways. Reference: n/a. Function: nan