ADP-ribosylation factor-like protein 4D (ARL4D), recombinant human

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-201aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: GNHLTEMAPT ASSFLPHFQA LHVVVIGLDS AGKTSLLYRL KFKEFVQSVP TKGFNTEKIR VPLGGSRGIT FQVWDVGGQE KLRPLWRSYT RRTDGLVFVV DAAEAERLEE AKVELHRISR ASDNQGVPVL VLANKQDQPG ALSAAEVEKR LAVRELAAAT LTHVQGCSAV DGLGLQQGLE RLYEMILKRK KAARGGKKRR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors GEF and GTPase-activating proteins GAP. GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form. Reference: n/a. Function: nan