Acyloxyacyl hydrolase (AOAH), recombinant human

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 35-575aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: LSNGHTCVGC VLVVSVIEQL AQVHNSTVQA SMERLCSYLP EKLFLKTTCY LVIDKFGSDI IKLLSADMNA DVVCHTLEFC KQNTGQPLCH LYPLPKETWK FTLQKARQIV KKSPILKYSR SGSDICSLPV LAKICQKIKL AMEQSVPFKD VDSDKYSVFP TLRGYHWRGR DCNDSDESVY PGRRPNNWDV HQDSNCNGIW GVDPKDGVPY EKKFCEGSQP RGIILLGDSA GAHFHISPEW ITASQMSLNS FINLPTALTN ELDWPQLSGA TGFLDSTVGI KEKSIYLRLW KRNHCNHRDY QNISRNGASS RNLKKFIESL SRNKVLDYPA IVIYAMIGND VCSGKSDPVP AMTTPEKLYS NVMQTLKHLN SHLPNGSHVI LYGLPDGTFL WDNLHNRYHP LGQLNKDMTY AQLYSFLNCL QVSPCHGWMS SNKTLRTLTS ERAEQLSNTL KKIAASEKFT NFNLFYMDFA FHEIIQEWQK RGGQPWQLIE PVDGFHPNEV ALLLLADHFW KKVQLQWPQI LGKENPFNPQ IKQVFGDQGG H. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria. Reference: n/a. Function: nan