ACSL3 (human, recombinant, aa 45-720)

Long-chain acyl-CoA synthetase 3 (ACSL3) is an enzyme involved in lipid biosynthesis and fatty acid degradation. It is composed of short N- and C-terminal domains surrounding an AMP-binding domain. It is expressed in the brain and prostate and localizes to the endoplasmic reticulum, mitochondria, and lipid droplets. ACSL3 is activated by a variety of factors, including peroxisome proliferator-activated receptor delta (PPARdelta), octamer-binding transcription factor 1 (Oct1), and the liver X receptor (LXR), among others. ACSL3 converts free monounsaturated long-chain fatty acids into fatty acyl-CoA esters, which are used as substrates for phospholipid and glycerolipid biosynthesis or transported into the mitochondria to be degraded via fatty acid beta-oxidation for use as an energy source. ACSL3-dependent conversion of exogenous MUFAs into fatty acyl-CoA esters can induce resistance to ferroptotic cell death by displacing PUFAs and preventing the accumulation of lipid reactive oxygen species (ROS) in the plasma membrane. ACSL3 is required for tumor cell survival in non-small cell lung cancer (NSCLC) mouse xenograft models, including those expressing K-Ras containing the glycine-to-aspartate activating mutation at position 12 (K-RasG12D). It is also highly expressed in tumor tissues isolated from patients with early-stage NSCLC. In contrast, homozygous deletion of ACSL3 is associated with an increased risk of metastasis or recurrence in patients with triple-negative breast cancer (TNBC) receiving adjuvant chemotherapy. Cayman's ACSL3 (human, recombinant) protein can be used for ELISA, enzyme activity assay, and Western blot applications.Synonyms: ACS3, Fatty Acid CoA Ligase 3, LACS 3, Long-chain Acyl-CoA Synthetase 3, Long-chain Fatty Acid-CoA Ligase 3. Purity: >90% estimated by SDS-PAGE. Source: Active recombinant human N-Terminal His-tagged ACSL3 expressed in E. coli. Amino Acids: 45-720. MW: 77.19 kDa. Formulation: (Request formulation change), 50 mM Tris pH 8.0, with 150 mM sodium chloride and 10% glycerol. UniProt Accession #: P33121.