Acrosin (ACR), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 20-343aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-Myc-tagged. Target Protein Sequence: KDNATCDGPC GLRFRQNPQG GVRIVGGKAA QHGAWPWMVS LQIFTYNSHR YHTCGGSLLN SRWVLTAAHC FVGKNNVHDW RLVFGAKEIT YGNNKPVKAP LQERYVEKII IHEKYNSATE GNDIALVEIT PPISCGRFIG PGCLPHFKAG LPRGSQSCWV AGWGYIEEKA PRPSSILMEA RVDLIDLDLC NSTQWYNGRV QPTNVCAGYP VGKIDTCQGD SGGPLMCKDS KESAYVVVGI TSWGVGCARA KRPGIYTATW PYLNWIASKI GSNALRMIQS ATPPPPTTRP PPIRPPFSHP ISAHLPWYFQ PPPRPLPPRP PAAQ. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome. Reference: "Mitochondrial functionality modifies human sperm acrosin activity, acrosome reaction capability and chromatin integrity." Zhang G., Yang W., Zou P., Jiang F., Zeng Y., Chen Q., Sun L., Yang H., Zhou N., Wang X., Liu J., Cao J., Zhou Z., Ao L. Hum. Reprod. 34:3-11(2019). Function: nan