a-Fetoprotein (aFP) Recombinant, Canine

Source:, Recombinant Canine from E. coli, Purity: >95%, Endotoxin: 1.0EU per 1ug (determined by the LAL method), Accession No: Q8MJU5, Fragment: Thr234~Leu380 (Accession No: Q8MJU5), Sequence: MGHHHHHHSGSEF- TFRAITV TKLSQKFSKA NFTEIQKLVL DVAHIHEECC RGNVLECLQD GEKIMSYICS QQDILSSKIA DCCKLPILEL GQCIIHAEND GKPEGLSPNL NRFLEERDFN QFSSREKDLF MARFTYEYSR RHTKLAVPVV LRVAKGYQEL, Epitope Tag: N-terminal His-Tag, Molecular Weight: 18.4kD, Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE., Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -80°C. Aliquots are stable for at least 12 months from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.