A disintegrin and metalloproteinase with thrombospondin motifs 14 (ADAMTS14), partial, human, recomb

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 253-555aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: HAKPGSYSIE VLLVVDDSVV RFHGKEHVQN YVLTLMNIVD EIYHDESLGV HINIALVRLI MVGYRQSLSL IERGNPSRSL EQVCRWAHSQ QRQDPSHAEH HDHVVFLTRQ DFGPSGYAPV TGMCHPLRSC ALNHEDGFSS AFVIAHETGH VLGMEHDGQG NGCADETSLG SVMAPLVQAA FHRFHWSRCS KLELSRYLPS YDCLLDDPFD PAWPQPPELP GINYSMDEQC RFDFGSGYQT CLAFRTFEPC KQLWCSHPDN PYFCKTKKGP PLDGTECAPG KWCFKGHCIW KSPEQTYGQD GGW. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Has aminoprocollagen type I processing activity in the absence of ADAMTS2. Seems to be synthesized as a latent enzyme that requires activation to display aminoprocollagen peptidase activity. Cleaves lysyl oxidase LOX at a site downstream of its propeptide cleavage site to produce a short LOX form. Reference: "Characterization of ADAMTS14, a novel member of the ADAMTS metalloproteinase family." Bolz H., Ramirez A., von Brederlow B., Kubisch C. Biochim. Biophys. Acta 1522:221-225(2001). Function: nan