7-dehydrocholesterol reductase (DHCR7), bovine, recombinant

Organism: Bos taurus (Bovine). Source: in vitro E.coli expression system. Expression Region: 1-475aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MAAKSQPSAP KTKSTSGLTN GNAAAQGQWG RAWEVDWFSL ASVIFLLLFA PFIVYYFIMA CDQYGCSLTV PVADLATGRA RLADIWARTP PVTAKAAQIY TAWVTLQVLL YMLLPDFCHK FLPGYVGGVQ EGAVTPAGAV NKYEINGLQA WLLTHLLWFA NAHLLGWFSP TIIFDNWIPL LWCANILGYT VSTFAMVKGY LFPTDARECK FTGNFFYNYM MGVEFNPRIG KWFDFKLFFN GRPGIVAWTL INLSFAAKQQ ELYGHVTNSM VLVNILQAIY VLDFFWNETW YLKTIDICHD HFGWYLGWGD CVWLPYLYTL QGLYLVYHPV QLPTYYALGV LLLGLLGYYI FRMTNHQKDL FRRTDGRCLI WGRKPKAIEC SYTSADGQRH HSKLLVSGFW GVARHFNYTG DLMGSLAYCL ACGGGHLLPY FYIIFMAILL THRCLRDEHR CANKYGRDWE HYTAAVPYRL LPGIF. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: 7-dehydrocholesterol reductase of the cholesterol biosynthetic pathway reducing the C7-C8 double bond of cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien-3beta-ol, two intermidiates in that pathway. Reference: "Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005). Function: nan