2-oxoglutarate dehydrogenase, mitochondrial (Ogdh),partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 695-1023aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: VDKRTCIPMN HLWPNQAPYT VCNSSLSEYG VLGFELGFAM ASPNALVLWE AQFGDFNNMA QCIIDQFICP GQAKWVRQNG IVLLLPHGME GMGPEHSSAR PERFLQMCND DPDVLPDLQE ENFDINQLYD CNWIVVNCST PGNFFHVLRR QILLPFRKPL IVFTPKSLLR HPEARTSFDE MLPGTHFQRV IPENGPAAQD PHKVKRLLFC TGKVYYDLTR ERKARNMEEE VAITRIEQLS PFPFDLLLKE AQKYPNAELA WCQEEHKNQG YYDYVKPRLR TTIDRAKPVW YAGRDPAAAP ATGNKKTHLT ELQRFLDTAF DLDAFKKFS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Reference: "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.Submitted (FEB-2005). Function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components