1-Phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (PLCZ1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-415aa. Protein Length: Full length of Isoform 2. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MEMRWFLSKI QDDFRGGKIN LEKTQRLLEK LDIRCSYIHV KQIFKTSDYP VVLSLENHCS TAQQEVMADN LQATFGESLL SDMLDDFPDT LPSPEALKFK ILVKNKKIGT LKETHERKGS DKRGDNQDKE TGVKKLPGVM LFKKKKTRKL KIALALSDLV IYTKAEKFKS FQHSRLYQQF NENNSIGETQ ARKLSKLRVH EFIFHTRKFI TRIYPKATRA DSSNFNPQEF WNIGCQMVAL NFQTPGLPMD LQNGKFLDNG GSGYILKPHF LRESKSYFNP SNIKEGMPIT LTIRLISGIQ LPLTHSSSNK GDSLVIIEVF GVPNDQMKQQ TRVIKKNAFS PRWNETFTFI IHVPELALIR FVVEGQGLIA GNEFLGQYTL PLLCMNKGYR RIPLFSRMGE SLEPASLFVY VWYVR. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating bryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete mbrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. Reference: Phospholipase Czeta causes Ca2+ oscillations and parthenogenetic activation of human oocytes.Rogers N.T., Hobson E., Pickering S., Lai F.A., Braude P., Swann K.Reproduction 128:697-702(2004). Function: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.