PFKL (Vector pENTR223.1, Accession No. BC008964)

Length: 2343 Sequence: atggccgcgg tggacctgga gaagctgcgg gcgtcgggcg cgggcaaggc catcggcgtc ctgaccagcg gcggcgacgc gcaaggcatg aacgctgctg tccgggctgt gacgcgcatg ggcatttatg tgggtgccaa agtcttcctc atctacgagg gctatgaggg cctcgtggag ggaggtgaga acatcaagca ggccaactgg ctgagcgtct ccaacatcat ccagctgggc ggcactatca ttggcagcgc tcgctgcaag gcctttacca ccagggaggg gcgccgggca gcggcctaca acctggtcca gcacggcatc accaacctgt gcgtcatcgg cggggatggc agccttacag gtgccaacat cttccgcagc gagtggggca gcctgctgga ggagctggtg gcggaaggta agatctcaga gactacagcc cggacctact cgcacctgaa catcgcgggc ctagtgggct ccatcgataa cgacttctgc ggcaccgaca tgaccatcgg cacggactcg gccctccacc gcatcatgga ggtcatcgat gccatcacca ccactgccca gagccaccag aggaccttcg tgctggaagt gatgggccgg cactgcgggt acctggcgct ggtatctgca ctggcctcag gggccgactg gctgttcatc cccgaggctc cacccgagga cggctgggag aacttcatgt gtgagaggct gggtgagact cggagccgtg ggtcccgact gaacatcatc atcatcgctg agggtgccat tgaccgcaac gggaagccca tctcgtccag ctacgtgaag gacctggtgg ttcagaggct gggcttcgac acccgtgtaa ctgtgctggg ccacgtgcag cggggaggga cgccctctgc cttcgaccgg atcctgagca gcaagatggg catggaggcg gtgatggcgc tgctggaagc cacgcctgac acgccggcct gcgtggtcac cctctcgggg aaccagtcag tgcggctgcc cctcatggag tgcgtgcaga tgaccaagga agtgcagaaa gccatggatg acaagaggtt tgacgaggcc acccagctcc gtggtgggag cttcgagaac aactggaaca tttacaagct cctcgcccac cagaagcccc ccaaggagaa gtctaacttc tccctggcca tcctgaatgt gggggccccg gcggctggca tgaatgcggc cgtgcgctcg gcggtgcgga ccggcatctc ccatggacac acagtatacg tggtgcacga tggcttcgaa ggcctagcca agggtcaggt gcaagaagta ggctggcacg acgtggccgg ctggttgggg cgtggtggct ccatgctggg gaccaagagg accctgccca agggccagct ggagtccatt gtggagaaca tccgcatcta tggtattcac gccctgctgg tggtcggtgg gtttgaggcc tatgaagggg tgctgcagct ggtggaggct cgcgggcgct acgaggagct ctgcatcgtc atgtgtgtca tcccagccac catcagcaac aacgtccctg gcaccgactt cagcctgggc tccgacactg ctgtaaatgc cgccatggag agctgtgacc gcatcaaaca gtctgcctcg gggaccaagc gccgtgtgtt catcgtggag accatggggg gttactgtgg ctacctggcc accgtgactg gcattgctgt gggggccgac gccgcctacg tcttcgagga ccctttcaac atccacgact taaaggtcaa cgtggagcac atgacggaga agatgaagac agacattcag aggggcctgg tgctgcggaa cgagaagtgc catgactact acaccacgga gttcctgtac aacctgtact catcagaggg caacggcgtc ttcgactgca ggaccaatgt cctgggccac ctgcagcagg gtggcgctcc aacccccttt gaccggaact atgggaccaa gctgggggtg aaggccatgc tgtggttgtc ggagaagctg cgcgaggttt accgcaaggg acgggtgttc gccaatgccc cagactcggc ctgcgtgatc ggcctgaaga agaaggcggc ggccttcagc cccgtcactg agctcaagaa agacactgat ttcgagcacc gcatgccacg ggagcagtgg tggctgagcc tgcggctcat gctgaagatg ctggcacaat accgcatcag tatggccgcc tacgtgtcag gggagctgga gcacgtgacc cgccgcaccc tgagcatgga caagggcttc Protein function: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway. [The UniProt Consortium]