LARGE (Vector pUC, Accession No. BC117425)

Length: 2271 Sequence: atgctgggaa tctgcagggg gagacggaaa ttcttggctg cctcgttgag tcttctctgc atcccagcca tcacctggat ttacctgttt tctgggagct tcgaagatgg aaagcccgtg tctctgtcac cgctggagtc ccaggcacac agccccaggt acacggcctc cagccagcgg gagcgcgaga gcctggaggt gcgcatgcgc gaggtggagg aggagaaccg cgccctccgc aggcagctca gcctggccca gggccgagcc ccatcccatc gccgaggcaa ccactccaag acctactcca tggaggaggg cactggagac agcgagaacc ttcgggctgg catcgtggca ggcaacagct ccgagtgtgg gcagcagccg gtcgtggaga aatgcgagac aatccacgtt gctattgtct gcgccggata caatgccagc cgggatgtcg tcaccctggt caaatccgtc ctgttccata gacggaaccc tctgcacttc caccttattg ctgactccat tgcggagcag atcctggcca cgctcttcca gacctggatg gtgcccgctg tgcgtgtgga cttctacaat gcagacgagc tcaagtctga agtttcctgg atccccaata aacattactc tgggatttat ggtctgatga agcttgtcct gaccaagact cttcctgcca acctggagag agtcatcgtc cttgacacgg atatcacctt tgccactgac attgcagagc tgtgggctgt gttccacaag ttcaaaggtc agcaagtcct gggcttggtg gagaaccaga gtgactggta ccttggaaac ctgtggaaaa atcaccgccc atggccagcc cttggaagag gctacaacac aggggtgatc ctgttacttc tggataagct gcggaagatg aaatgggagc agatgtggag gctgaccgca gagagggagc tcatgggcat gctctctaca tccttagctg accaggatat tttcaatgcc gtcatcaaac aaaacccctt ccttgtgtac cagctcccct gcttctggaa tgtgcagctg tcagaccaca cccgctccga gcagtgctac agagacgtgt ctgatctaaa ggtcattcac tggaactccc ccaagaagct ccgggtgaag aacaagcatg tggagttttt tcgcaacctc tacctgacct tcctggagta tgacggcaat cttctgaggc gggaactgtt tggctgcccc agtgaggctg atgtcaacag tgaaaacctc cagaagcagc tgtctgagct ggacgaggac gacctgtgct atgagttccg gcgagagcgc ttcactgtcc accgcaccca cctgtacttc ctgcactacg agtatgagcc tgcagcagac agcacggacg tcaccctggt cgctcagctg tccatggaca ggctccagat gctggaggcc atctgcaagc actgggaggg gcccatcagc ctggccctct acctgtcaga cgccgaggcc cagcagttcc tccgctacgc acagggctct gaggtgctta tgagccgcca caacgtgggc taccacatcg tgtacaagga gggccagttc taccccgtga acctgctgcg caacgtggcc atgaagcaca tcagcactcc ctacatgttc ctgtctgaca ttgacttcct gcccatgtat gggctctatg agtacctcag gaagtctgtc atccagctcg atcttgccaa caccaagaaa gcaatgattg tccccgcgtt cgagacactg cgctaccggc tgtccttccc caagtcaaaa gcggagttgc tgtcaatgct ggacatgggg accctcttca cattcaggta ccacgtctgg acgaaaggcc acgcacccac aaacttcgcc aagtggcgga ccgccaccac gccttaccgg gttgagtggg aggccgattt tgagccgtat gttgttgtga gacgtgactg cccggagtac gaccggaggt ttgtaggctt tggctggaac aaagtggctc atatcatgga gctggatgtg caggagtatg agttcattgt gctgcccaac gcctacatga tccacatgcc tcatgccccc agcttcgaca ttaccaagtt ccgttccaac aagcaatacc gcatctgtct caaaaccctc aaggaagagt ttcagcagga catgtcccgc cgctacggct ttgctgccct gaaatatctc acagccgaga acaacagcta Protein function: Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N- acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate- 6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:22223806). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4- xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed:25279699). [The UniProt Consortium]