FNIP1 (Vector pENTR223.1, Accession No. BC001956)

Length: 1527 Sequence: atggccccta cgctgttcca gaagctcttc agcaagagga ccgggctggg cgcgcccggc cgcgacgccc gggacccaga ttgcgggttc agttggcctt taccagagtt tgatccaagc cagattcgac tgattgtata tcaagactgt gaaagacgag ggagaaatgt tttgtttgac tccagtgtta agagaagaaa tgaggacata tcagtatcga aactctgcag tgatgctcaa gttaaagtct ttgggaaatg ctgccaactg aaacctggag gagacagttc ttcctcttta gatagttctg tgacttcatc ttctgatata aaagaccagt gtcttaagta ccagggttct cggtgctctt ctgatgccaa tatgcttgga gagatgatgt ttggctcagt agcaatgagc tacaaaggat ccaccttaaa aattcatcag attcgttccc ctccacagct catgctcagc aaagtgttta ctgctcggac tggcagcagt atttgtggga gtctcaatac gctacaagat agtcttgaat tcatcaatca ggacaacaat acattaaagg ctgataataa cacagttatt aatggactgc ttggaaatat aggtctttca cagttctgca gccccaggcg ggcattctct gagcagggtc cgctccgcct gatcaggagc gcctctttct ttgcagttca cagcaaccca atggacatgc ctggaagaga gctcaatgag gacagagaca gtggcatagc acggtctgca tctctcagca gcttgctgat cactccattt ccttccccaa actcctcact tacccgaagt tgtgccagca gctaccagcg acgttggcga cgcagccaaa caacaagttt ggaaaatggg gtatttccta gatggtctat agaagaaagc tttaatctct cagatgaaag ctgtggccct aacccaggaa ttgtgcggaa aaagaagatt gcaattgggg taatcttttc attgtccaaa gatgaagatg aaaataacaa atttaatgaa ttcttttttt cacattttcc tctctttgaa agccacatga acaaattaaa gagtgcaata gaacaggcta tgaaaatgag ccggagatca gctgatgcca gtcagagaag tttggcatat aatcgaatag ttgatgccct aaatgaattc agaacaacaa tttgtaatct ttacacgatg ccacgaattg gagaacctgt ctggcttaca atgatgtcgg ggactccaga aaagaaccac ctttgctatc gtttcatgaa ggagttcacc tttctaatgg aaaatgcttc caaaaatcaa ttcttgccag ctctcattac tgcagttctg accaatcatc ttgcctgggt tccaacagtc atgccaaatg gacaaccacc tataaaaata tttttagaaa agcattcctc tcagagtgtg gacatgttgg caaagactca tccatataac ccactttggg cacaactggg Protein function: Binding partner of the GTPase-activating protein FLCN: involved in the cellular response to amino acid availability by regulating the mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3 (PubMed:17028174, PubMed:18663353, PubMed:24081491). In low-amino acid conditions, component of the lysosomal folliculin complex (LFC) on the membrane of lysosomes, which inhibits the GTPase- activating activity of FLCN, thereby inactivating mTORC1 and promoting nuclear translocation of TFEB and TFE3. Upon amino acid restimulation, disassembly of the LFC complex liberates the GTPase- activating activity of FLCN, leading to activation of mTORC1 and subsequent cytoplasmic retention of TFEB and TFE3. Required to promote FLCN recruitment to lysosomes and interaction with Rag GTPases (PubMed:24081491). Together with FLCN, regulates autophagy: following phosphorylation by ULK1, interacts with GABARAP and promotes autophagy (PubMed:25126726). In addition to its role in mTORC1 signaling, also acts as a co-chaperone of HSP90AA1/Hsp90: following gradual phosphorylation by CK2, inhibits the ATPase activity of HSP90AA1/Hsp90, leading to activate both kinase and non-kinase client proteins of HSP90AA1/Hsp90 (PubMed:27353360, PubMed:30699359). Acts as a scaffold to load client protein FLCN onto HSP90AA1/Hsp90 (PubMed:27353360). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360). Required for B-cell development. [The UniProt Consortium]