FADS3 (Vector pUC, Accession No. BC004901)

Length: 1338 Sequence: atgggcggcg tcggggagcc gggaccgcgg gagggacccg cgcagccggg ggcaccgctg cccaccttct gctgggagca gatccgcgcg cacgaccagc ccggcgacaa gtggctggtc atcgagcgcc gcgtctacga catcagccgc tgggcacagc ggcacccagg gggcagccgc ctcatcggcc accacggcgc tgaggacgcc acggatgcct tccgtgcctt ccatcaagat ctcaattttg tgcgcaagtt cctacagccc ctgttgattg gagagctggc tccggaagaa cccagccagg atggacccct gaatgcgcag ctggtcgagg acttccgagc cctgcaccag gcagccgagg acatgaagct gtttgatgcc agtcccacct tctttgcttt cctactgggc cacatcctgg ccatggaggt gctggcctgg ctccttatct acctcctggg tcctggctgg gtgcccagtg ccctggccgc cttcatcctg gccatctctc aggctcagtc ctggtgtctg cagcatgacc tgggccatgc ctccatcttc aagaagtcct ggtggaacca cgtggcccag aagttcgtga tggggcagct aaagggcttc tccgcccact ggtggaactt ccgccacttc cagcaccacg ccaagcccaa catcttccac aaagacccag acgtgacggt ggcgcccgtc ttcctcctgg gggagtcatc cgtcgagtat ggcaagaaga aacgcagata cctaccctac aaccagcagc acctgtactt cttcctgatc ggcccgccgc tgctcaccct ggtgaacttt gaagtggaaa atctggcgta catgctggtg tgcatgcagt gggcggattt gctctgggcc gccagcttct atgcccgctt cttcttatcc tacctcccct tctacggcgt ccctggggtg ctgctcttct ttgttgctgt cagggtcctg gaaagccact ggttcgtgtg gatcacacag atgaaccaca tccccaagga gatcggccac gagaagcacc gggactgggt cagctctcag ctggcagcca cctgcaacgt ggagccctca cttttcacca actggttcag cgggcacctc aacttccaga tcgagcacca cctcttcccc aggatgccga gacacaacta cagccgggtg gccccgctgg tcaagtcgct gtgtgccaag cacggcctca gctacgaagt gaagcccttc ctcaccgcgc tggtggacat cgtcaggtcc ctgaagaagt ctggtgacat ctggctggac gcctacctcc Protein function: Mammals have different sphingoid bases that differ in their length and/or pattern of desaturation and hydroxyl groups. The predominant sphingoid base in mammalian ceramides is sphing-4-enine (sphingosine or SPH) which has a trans desaturation at carbon 4. FADS3 is a ceramide desaturase that introduces a cis double bond between carbon 14 and carbon 15 of the SPH-containing ceramides, producing sphinga-4,14-dienine-containing ceramides (SPD ceramides). SPD ceramides occur widely in mammalian tissues and cells. Due to their unusual structure containing a cis double bond, SPD ceramides may have an opposite, negative role in lipid microdomain formation relative to conventional ceramides (PubMed:31916624). FADS3 also acts as a methyl- end fatty acyl coenzyme A (CoA) desaturase that introduces a cis double bond between the preexisting double bond and the terminal methyl group of the fatty acyl chain. Desaturates (11E)-octadecenoate (trans- vaccenoate, the predominant trans fatty acid in human milk) at carbon 13 to generate (11E,13Z)-octadecadienoate (also known as conjugated linoleic acid 11E,13Z-CLA). [The UniProt Consortium]